Self-assembly of proximity probes for flexible and modular proximity ligation assays

Self-assembly of proximity probes for flexible and modular proximity ligation assays.

Proximity ligation assay (PLA) is a recently developed strategy for protein analysis in which antibody-based detection of a target protein via a DNA ligation reaction of oligonucleotides linked to the antibodies results in the formation of an amplifiable DNA strand suitable for analysis. Here we describe a faster and more cost-effective strategy to construct the antibody-based proximity ligatio...

Improvements and Applications of in situ Proximity Ligation Assays

Grannas, K. 2015. Improvements and Applications of in situ Proximity Ligation Assays. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1099. 48 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9233-5. The cells building up the human body is in constant communication with each other. This communication is done through large complex networks of sig...

Development and Application of Triple Specific Proximity Ligation Assays (3PLA)

Best proximity point theorems in 1/2−modular metric spaces

‎In this paper‎, ‎first we introduce the notion of $frac{1}{2}$-modular metric spaces and weak $(alpha,Theta)$-$omega$-contractions in this spaces and we establish some results of best proximity points‎. ‎Finally‎, ‎as consequences of these theorems‎, ‎we derive best proximity point theorems in modular metric spaces endowed with a graph and in partially ordered metric spaces‎. ‎We present an ex...

Homogeneous and digital proximity ligation assays for the detection of Clostridium difficile toxins A and B

BACKGROUND The proximity ligation assay (PLA) detects proteins via their interaction with pairs of proximity probes, which are antibodies coupled to noncomplementary DNA oligonucleotides. The binding of both proximity probes to their epitopes on the target protein brings the oligonucleotides together, allowing them to be bridged by a third oligonucleotide with complementarity to the other two. ...